P-chlorobenzamidoacetohydroxamic acid

ABSTRACT

WHEREIN R is nitro or chloro are potent inhibitors of urease. Combined with urinary tract antibacterials an improvement in the prevention of formation of urinary calculi in the presence of urea splitting organisms is observed.   Novel benzamidoacetohydroxamic acids of the formula:

United States Pilllel lt [191 Johnson et al,

[ 1 Apr. 17,1973

[ 1 R-CHLOROBENZAMIDO ACETOHYDROXAMIC ACID [75] Inventors: Roland N, Johnson; Jon A. Andersen, both of Norwich, NY.

[73] Assignee: Morton-Norwich Products, Inc.,

' Norwich, NY.

[22] Filed: May 17, 1971 [21] App1.'No.: 144,322

[52] US. Cl ..260/500.5 H, 424/324 3,634,509 1/1972 Yates et a1 ..260/500.5 H

3,444,232 5/1969 Bernstein .....260/500.5H

FOREIGN PATENTS OR APPLICATIONS 1,048,936 11/1966 Great Britain ..260/500.5H

Primary Examiner-Leon Zitver Assistant Exa'miner Joseph Evans AttorneyBradford S. Allen 1 ABSTRACT Novel ben'zamidoacetohydroxamic acids of the forinii- 1a: v

wherein R is nitro or chloro are potent inhibitors of urease. Combined withrui-inary tract antibacterials an improvement in the prevention of formation ofnrinary calculi in .the presence of urea splitting organisms is observed.

' 1 Claim, No Drawings P-CHLOROBENZAMIDOACETOHYDROXAMIC ACID This invention is concerned with chemical compounds. -More particularly it is concerned with compounds of the formula:

RQ-CONHCHKJONHOH wherein R is nitro or chloro and their combination with urinary tract antibacterial agents.

' The compounds of formula 1 are potent urease inhibitors. They may be readily prepared according to this scheme:

ROONHCHCONIIOH 0.115011 wherein R is nitro or chloro. Exemplary of this scheme are the following:

I I Example I 2-(p-Nitrobenzamido)acetohydroxamic Acid .trate was acidified with glacial acetic acid to pH 6,v

precipitating a white crystalline solid which was dried at 60 to 38 g. The two crops were combined with g.

from a similar run and recrystallized from methanol to give 69 g. (67 percent), m.p. l60--.1.

" Calcd. for c,11,,N o',; c, 45.19; 11, 3.79; N, 17.57

Found: c, 45.10; H, 3.80; N, 17.53

Example 2 2-(p-Chlorobenzamido)acetohydroxamic Acid Into a suspension of 209 g. (1.50 moles) of glycine ethyl ester hydrochloride in 1,200 ml. of dry benzene was slowly added 263 g. (1.50 moles) of pchlorobenzoyl chloride. This mixture was heated under reflux for 22 hours, then filtered and the white precipitate was recrystallized frommethanol giving 292 g.(82.5%) of ethyl 2-(p-chlorobenzamido)acetate, m.p. l14-117C.

-Anal. Calcd. for C H NO CI (241.67): C, 54.67; H,

5.01; N, 5.80 Found: C, 54.95; H, 4.97; N, 6.08

To a stirred solution of 58 g. (0.83 mole) of hydroxylamine hydrochloride in 350 ml. of anhydrous methanol was added a solution of 70 g. of potassium hydroxide in 150 ml. of anhydrous methanol. A white salt immediately precipitated. This mixture was cooled in an ice bath and the salt was removed by filtration.

The filtrate was placed into a 2 1. flask equipped with a stirrer, a thermometer and a drying tube. To the filtrate was added with stirring g. (0.42 mole) of ethyl 2- (p-chlorobenzamido)acetate which went into solution. Approximately Zminutes later a thick white precipitate formed. The mixture was stirred at room temperature overnight. The potassium salt was collected by filtration and dried at 60to 90 g.

A second run of the same size was made with 91 g. of the potassium salt being obtained. The two crops of salt were combined making a total of 181g. which was placed in 2,000 ml. of cold water. Glacial acetic acid (40 ml.) was added to give a final pH of 5. The white precipitate was collected by filtration and dried to g. in a 60 oven. The 160 g. of crude product was recrystallized from 4,800 ml. of methanol. After cooling the solution overnight in the refrigerator, the product precipitated as white crystals which'were collected by filtration and dried at 60C to 121 g.(63.8 percent overall yield), m.p. 171 172C.

The anti-urease activity of the compounds of this invention is exemplified below:

TABLE I The Inhibition of Urease purified from Proteus miralrilis (Pr-91 and of Urease Contained Within Intact Cells of Several Species and Strains of Proteus Concentration of Inhibitor for 50% Inhibition of Urease Compound of Ex. 1 Compound of Ex. 2* mol/l. ug/l. mole/l. ug/l. Purified'Enzyme Proteus mirabilis' Pr-9l 5.3X l0' 128 ZLSXIO 57 Intact Cells I i Proteus mirabilis I i Pr+9l LIXlO" 264' 3.6X10' 82 Pr-l04 l.3XlO' 310 4.9Xl0 Pr-l05 7.4Xl0 178 3.6)(10 I 82 Pr-92 2.2X10 526 3.9Xl0'7 89 v Proteus vulgan's 2.9700" 69 3.6;)(10'7 40x10 96 3.5x10- so Proteus 'morgam'i Pr-94 1.2x10- 286 2.9Xl0' 66 Pr-lOO 6.2Xl0" i458 1.5)(10' 342 I Pr-lOl 7.7)(10 l84 3L3Xl0' 75 The compounds of this invention are valuable adjuncts in the therapy of urinary tract infection caused by urea splitting bacteria such as Proteus mirabilis whereby urea is decomposed to ammonia with resultant formation of insoluble salts such as ammonium magnesium phosphate giving rise to calculi formation and obstruction posing a site of reinfection impairing the efficacy of otherwise efiective urinary tract a'ntibacterials such as ampicillin, sulfamethoxazole and nitrofurantoin.

When the compounds of .this invention are administered in conjunction with known antibacterial' lUulcull in mtsnitcr 7 days infection with Imlnlm mimhilia (lr !|I)] Statistical significance 1 ol the dificrcncc Mean mg. between an Test for Rats phosphorus experimental potentiation 1 Dosage, per as calculi and the con Compound(s) mgJkg. group per bladder trol group EU AB None 50 5. 4

12.5 10 1.!) 0. 01 12.5 i) 1.1 0.05 2.5 i) 2.2 0.02 25 10 3. 3 0. 02 50 i) 1. 0.01 25 I s 3.3 0.05 50 7 2. 3 0. 02 Compound of Example 1 5 5 1 plus sulinnregioxazolle.1.... 5 I l 0.3 0. 0i

jompoun o xamp e C lus sultgm eggwxazo leuhl g s) 0. ii 0.01 0.01 0.01

ompoun o xampe2 plus suligmfoghoxazollmung 2'} 'i 0. u U. 01 0.01 0. 01 Zompoun 0 uxamp c 2 mus s'ulfi1ir1o%hoxnz0llc f: u 0.1; o. 01 0. m 0.01

)ompoun 0 xampel lus niti;fii}r%nt0in l 1 g 0 a 0.01 0.01 n.0i

ompoun o xamp o plus nitigiiirpzntohnfnzung 12 g 10 l .1 0.01 010 010 ,ompoun o xamp e Cplus Illtl;)fl}r%nt0lll.l ..2.. 2 l 0.1 0.01 0.01 0.01

ompoun 0 xamp e 1' plus nitrofurantoin g 10 0.8 out o. 01 0.01 Compound of Example 1 I Cplus am ici ilg nun 12 5 10 0.1 0. 01 n. 01 0. c1

ompoun o xamp e plus ampicillin g 0. 2 0 10 0.10 0.10 Compound 01' Example 2 plus ampicillin 12 5 9 0.1 0.01 0.01 0.01 Compound of Example 2 plus ampicillin 12 5 10 0.1 0.10 0. 01 o. 01

" Statistical evaluation was by the Wilcoxon rank sum test [Wilcoxon et al. "Some Rapid Approximate Statistical Procedures, Lederle Laboratories, 7-!) (1964)].

2 The number is the probability that the diflcrence in the mean mg. phosphorus as cystic culcull between rats receiving an antibacterial agent (AB) only or an ureasc inhibit-or (EU) only on the one hand, and the mean (mg.) phosphorus as cystic calculi from rats receiving both (AB) and (EU) on the other, is a chance occurrence.

' NOTE.Compounds or combinations of compounds were administered t.i.d. at the indicated dosages for 6 days beginning 24 hours after infection.

The compounds of this invention by themselves and in conjunction with ampicillin, sulfamethoxazole or nitrofurantoin are readily formulated in pharmaceutical dosage forms such as tablets, lozenges, suspensions, troche's, and capsules using conventional pharmaceuti- 

